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KMID : 0379520060220030229
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한국독성학회지
2006 Volume.22 No. 3 p.229 ~ p.235
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Anti-oxidative, Nitric Oxide Inhibitory Activities and Irritation Test of the Fermented Opuntia humifusa Cladodes
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Chang Zhi-Qiang
Hwang Mi-Hyun
Oh Byoung-Chol
Lee Sam-Pin
Rhee Man-Hee
Kim Kil-Soo
Jeong Kyu-Shik
Kim Jong-Choon
Park Seung-Chun
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Abstract
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Opuntia humifusa is a member of the Cactaceae family. In the present study, the antioxidant, nitric oxide(NO) inhibitory activities and potential irritation response of the fermented Opuntia humifusa cladodes(FOH) were investigated for cosmetic use. Antioxidant activities were tested using 1,1-diphenyl-2-picrylhydrazyl(DPPH) and xanthine oxidase assay, we found that FOH could scavenge DPPH free radicals and inhibit xanthine oxidase activity in a dose dependent manner, with IC_{50} of 2599.46{mu}g/ml and 721.38{mu}g/ml, respectively. To investigate the possible anti-inflammatory effects of FOH, RAW 264.7 macrophages were pretreated with FOH(0{sim}400{mu}g/ml) for 30 min and then treated with LPS for 24 h. We found that cell number did not vary significantly with the treatment of FOH, and FOH did not show any inhibitory effects on LPS-induced NO production. After application of FOH to rabbits for skin and eye irritation test, the experimental sites did not show any response compared to the control. FOH were considered to be a non-irritant to the skin and eye. Based on the above information, we suggest that FOH can be considered to be a non-irritant base cosmetic material for safely use.
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KEYWORD
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Opuntia humifusa, DPPH, Xanthine oxidase, Nitric oxide, Irritation
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